The gelling characteristics of two vicilin fractions from pea (Pisum sativum L.) were compared over a range of pH and salt conditions after preliminary results showed that despite having equal opportunity to unfold, and expose hydrophobic residues, they had different minimum gelling concentrations (at pH 7.6). Furthermore, at this pH one fraction formed turbid gels and the other formed transparent gels. The fraction that formed transparent gels contained a substantial amount of the 70 kDa alpha-subunits of vicilin, and thus it was hypothesized that the highly charged N-terminal extension region on these 70 kDa subunits hinders gelation of this vicilin fraction at pH 7.6 and I = 0.2 due to repulsion of the net negative charge. The experiments designed to test this hypothesis are presented and discussed in this paper and prove that the hypothesis was true, which offers the possibility to control or modify the gelation behavior of vicilin on the basis of information of its subunit composition.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf035105a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Designing & optimisation of dual Ca and SO ionic cross-linked sericin/pectin microbeads using response surface methodology for colon-specific delivery.
J Biomater Sci Polym Ed
January 2025
School of Studies in Pharmaceutical Sciences, Jiwaji University, Gwalior, India.
Ulcerative colitis, a chronic inflammatory condition of the colon, requires precise and targeted treatment, and polysaccharides, with their pH responsiveness and biodegradability, offer an innovative approach for colon-specific drug delivery. This study aims to develop a highly precise drug delivery system with enhanced therapeutic and targeting efficiency for ulcerative colitis, focusing on the preparation, optimisation, and evaluation of dual cross-linked mesalamine-loaded sericin-pectin (DSPs) micro-beads. These beads utilise the pH-responsive and microflora biodegradability properties of polysaccharides for targeted colon delivery, employing the Response Surface Methodology.
View Article and Find Full Text PDFFast fabrication of stimuli-responsive MXene-based hydrogels for high-performance actuators with simultaneous actuation and self-sensing capability.
J Colloid Interface Sci
January 2025
Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037 China; College of Chemical Engineering, Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, Nanjing Forestry University, Nanjing 210037 China. Electronic address:
Poly(N-isopropylacrylamide) (PNIPAM) composite hydrogels have recently emerged as promising candidates for soft hydrogel actuators. However, developing a facile and fast method to obtain multifunctional PNIPAM hydrogel actuators with simulating biological versatility remains a major challenge. Herein, we developed a fast-redox initiation system to prepare PNIPAM/sodium carboxymethyl cellulose (CMC)/TCT MXene nanocomposite hydrogel with multidirectional actuating behaviors and improved mechanical properties.
View Article and Find Full Text PDFGelatins derived from aronia-supplemented fish diets: Structural effect and molecular simulation.
Int J Biol Macromol
January 2025
Department of Biotechnological Genetics, Institute of Science, Trakya University, Edirne, Turkey.
Fish gelatin, a sustainable substitute for mammalian gelatin, frequently exhibits weaker gel strength and thermal stability, limiting its industrial uses. This study investigated an in vivo method to improve functional characteristics by supplementing Nile tilapia diets with Aronia extract. The control diet (A0) contained no Aronia extract, while the remaining four diets consisted of commercial pelleted feed enriched with 250 mg/kg (A250), 500 mg/kg (A500), 750 mg/kg (A750), and 1000 mg/kg (A1000) of Aronia extract.
View Article and Find Full Text PDFMolecular mechanism underlying the modulation of typical properties of starch by Quillaja saponins: Multi-experimental evidence and two-stage MD simulation.
Int J Biol Macromol
January 2025
College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China. Electronic address:
Quillaja saponins (QS), a natural amphiphilic food additive, have significant potential in modulating the properties of starchy products. However, a systematic understanding of this phenomenon and the underlying molecular mechanisms remains lacking. In this study, two-stage molecular dynamics (MD) simulations combined with multiple experimental approaches were employed to investigate the modulation of starch properties by QS through six chain dynamic behaviors.
View Article and Find Full Text PDFINVITED REVIEW: Recent developments in understanding the rehydration characteristics of high-protein dairy powders.
J Dairy Sci
January 2025
Midwest Dairy Foods Research Center, Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55128. Electronic address:
The use of dairy-based ingredients is increasingly prominent in the food industry due to their functional and nutritional benefits. High-protein powders are highly attractive due to their superior nutritional (e.g.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!