AI Article Synopsis
- Adenine nucleotides interact with specific receptors on cell surfaces, leading to various biological responses, particularly in neuroepithelial bodies (NEB) of neonatal hamster lungs, which are thought to sense oxygen levels.
- Immunohistochemistry showed that NEB cells express P2X receptors (specifically P2X2 and P2X3) alongside serotonin, indicating their role as functional receptors in oxygen sensing.
- Electrophysiological studies demonstrated that ATP causes an inward current in NEB cells, impacting serotonin release under hypoxic conditions, suggesting these receptors may help with the transmission of hypoxia signals.
Article Abstract
Adenine nucleotides act through specific cell surface receptors to invoke a variety of biological responses. Here we show that cells of neuroepithelial bodies (NEB), presumed O2 airway sensors in neonatal hamster lung, express functional P2X receptors (P2X-R). Positive immunostaining was detected in NEB cells using double-label immunohistochemistry with antibodies against P2X2 and P2X3 receptor subunits, which co-localized with serotonin (5-HT), a marker of NEB cells. For electrophysiological characterization of P2X2-R in NEB cells, fresh neonatal hamster lung slice preparation was used. Under whole-cell patch clamp, perfusion with ATP induced a concentration-dependent, non-desensitizing inward current (EC50=12 microM). Perfusion with alpha,beta-methylene ATP also induced a slow-desensitizing inward current (EC50=8.2 microM). Suramin (IC50 ca. 43 microM) and TNP-ATP (IC50 ca. 8 microM) blocked the currents evoked by both ATP and alpha,beta-methylene ATP. Using carbon fiber amperometry we observed that hypoxia and ATP induced 5-HT release from NEB cells and that this release was blocked by suramin. These data suggest that functional P2X2/3 heteromeric receptors are expressed in NEB cells. The possible function of these purinoreceptors in NEB cells could include modulation of hypoxia chemotransmission.
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| http://dx.doi.org/10.1515/BC.2004.022 | DOI Listing |
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