AI Article Synopsis
- Many eukaryotic transcripts, including human mRNAs, contain sequences that resemble ribosomal RNAs, with potential implications for regulating translation.
- Despite initial findings suggesting that long rRNA-like sequences in mRNA might be artifacts, a detailed analysis showed that shorter 9-nucleotide sequences related to 18S rRNA are unevenly represented in transcripts.
- This uneven representation indicates these sequences could play a role in post-transcriptional regulation of gene expression.
Article Abstract
Many eukaryotic transcripts have been reported to contain sequences similar or complementary to ribosomal RNAs. Past computational and experimental analyses have suggested a possible functional role of such rRNA-like sequences in the regulation of translation. Here, we analysed the occurrence and abundance of rRNA-like sequences in human transcripts. Using the wealth of data from genome and EST sequences, our study indicates that mRNA sequences with long highly similar rRNA-like sequences are most likely artifacts. In contrast, analysis of the occurrence of 9-nt sequences covering the entire direct and complementary 18S human rRNA sequence, revealed that many of them are significantly over- or underrepresented, suggesting their possible involvement in post-transcriptional regulation of gene expression.
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