AI Article Synopsis
- An automated immunoassay was developed to detect nicarbazin residues in poultry eggs and liver, using a novel dry chemistry approach and time-resolved fluorometry.
- The assay provides results in 18 minutes, making it a rapid and straightforward method suitable for high throughput screening.
- It has low limits of detection (0.1 ng/mL) with excellent sample recovery rates (97.3% to 115.6%) and minimal variations in results.
Article Abstract
An automated immunoassay for the detection of nicarbazin residues in poultry eggs and liver was developed. The assay was based on a novel all-in-one dry chemistry concept and time-resolved fluorometry. The analyte specific antibody was immobilized into a single microtiter well and covered with an insulation layer, on top of which the label was dried in a small volume. The extracted sample was added automatically to the dry microtiter well, and the result was available within 18 min. Due to the rapidity and simplicity, the quantitative immunoassay could also be used as a high throughput screening method. The analytical limit of detection for the assay was calculated as 0.1 ng mL(-)(1) (n = 12) and the functional limit of detection as 3.2 ng g(-)(1) for egg (n = 6) and 11.3 ng g(-)(1) for liver (n = 6) samples. The sample recovery varied from 97.3 to 115.6%. Typically, the intra-assay variations were less than 10%, and interassay variations ranged between 8.1 and 13.6%.
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