AI Article Synopsis
- The study aimed to compare two methods of analyzing urine sediment—unstained wet mounts and air-dried modified Wright-stained preparations—against standard bacteriologic culture in dogs.
- Both methods were evaluated for their effectiveness, with the modified Wright-stained preparation showing notably better sensitivity and specificity compared to the unstained method.
- Overall, the modified Wright-stained analysis demonstrated itself as a more reliable and efficient approach for detecting bacteriuria in canine urine samples.
Article Abstract
Objective: To compare the findings of light microscopic evaluation of routine unstained wet-mounted preparations and air-dried, modified Wright-stained preparations of urine sediment with results of quantitative aerobic bacteriologic culture of urine.
Design: Masked prospective study.
Sample Population: 459 urine samples collected by cystocentesis from 441 dogs.
Procedure: Urinalyses and quantitative bacteriologic cultures of urine were performed. Unstained wet-mounted preparations and air-dried, modified Wright-stained urine sediment preparations were examined by light microscopy for the presence of bacteria.
Results: Compared with results of quantitative bacteriologic culture, routine unstained preparations and modified Wright-stained preparations had sensitivities of 82.4% and 93.2%, specificities of 76.4% and 99.0%, positive predictive values of 40.1% and 94.5%, negative predictive values of 95.8% and 98.7%, and test efficiencies of 77.3% and 98.0%, respectively. Compared with 74 samples that yielded growth on bacteriologic culture, the routine unstained method had concordance and misclassification rates of 39.2% and 60.8%, respectively, whereas the Wright-stained method had concordance and misclassification rates of 78.4% and 21.6%, respectively. Significant associations between each of occult blood in urine, pyuria, female sex, and lower urine specific gravity with bacteriuria detected by Wright-stained sediment examination and quantitative bacteriologic culture of urine were identified.
Conclusions And Clinical Relevance: Examination of modified Wright-stained preparations of urine sediment appeared to be a rapid, cost effective method that significantly improved the sensitivity, specificity, positive predictive value, and test efficiency of light microscopic detection of bacteriuria, compared with that of the routine unstained method.
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| http://dx.doi.org/10.2460/javma.2004.224.1282 | DOI Listing |
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