AI Article Synopsis
- CEACAM1 is a cell adhesion protein found in epithelial and hematopoietic cells, with four major isoforms that have different cytoplasmic domain lengths.
- In a breast epithelial cell model, CEACAM1 is crucial for forming lumens.
- Two soluble isoforms from myeloma cells were found to be immunologically active and significantly inhibited lumen formation in this 3D model system.
Article Abstract
Carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is a type 1 transmembrane, homotypic cell adhesion protein expressed on epithelial and hematopoietic cells. CEACAM1 has four major isoforms with three or four immunoglobulin (Ig)-like ectodomains and either long or short cytoplasmic domains. In a 3D model of breast epithelial cell morphogenesis, CEACAM1 plays an essential role in lumen formation [J. Cell Sci. 112 (1999) 4193]. Two soluble ectodomain isoforms of CEACAM1 expressed in myeloma cells were immunologically active and highly glycosylated. The molecular weights of the 3-ecto- and 4-ectodomain isoforms were 90 and 110kDa, respectively, and monomers by sedimentation equilibrium centrifugation. Both isoforms were prolate ellipsoids with axial ratios of 6 for the 3-ecto- and 8 for 4-ectodomain isoforms, respectively, by size exclusion chromatography and analytical ultracentrifugation. Both isoforms caused a significant reduction in lumen formation when tested in the 3D model culture system.
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| http://dx.doi.org/10.1016/j.bbrc.2004.04.024 | DOI Listing |
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