AI Article Synopsis
- A new method for preparing human islets for transplantation was developed, combining collagenase digestion with a density gradient purification system.
- This technique achieved over 200,000 islet equivalents with more than 50% purity in 69% of processed glands, without using any animal-derived reagents.
- Isolated islets maintained their morphology and function, showing sterility in 97% of cases, and successful transplants were performed in two patients, demonstrating temporary graft function.
Article Abstract
We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (>/=200,000 islet equivalents, >/=50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method.
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| http://dx.doi.org/10.1016/j.transproceed.2004.02.047 | DOI Listing |
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