PCR standardization was performed in order to detect a fragment of bexA gene, which is presented in all capsulate H. influenzae isolates, and a DNA fragment specific for H. influenzae type b. Implementing of such PCR into H. influenzae typing may be very useful in cases of isolates from clinical material for which serotyping alone gives unclear results. Standardization of PCR detecting DNA fragments specific for all capsular types will enable to perform complete typing of H. influenzae isolates.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
High Antimicrobial Susceptibility of Cloacal Enterococci and from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing ST69.
Antibiotics (Basel)
January 2025
SaBio Health and Biotechnology Research Group, Institute for Game and Wildlife Research (IREC), Ronda de Toledo 12, 13071 Ciudad Real, Spain.
In 2022, an outbreak of H5N1 highly pathogenic avian influenza (HPAI) killed 60% of the largest breeding colony of Dalmatian pelicans (DPs) in the world at Mikri Prespa Lake (Greece), prompting a multidisciplinary study on HPAI and other pathogens. This study determines the antimicrobial resistance rates of cloacal enterococci and in DPs. Fifty-two blood and cloacal swab samples were collected from 31 nestlings (20 DP/11 great white pelicans) hatched after the H5N1 outbreak at the Prespa colony and 21 subadult/adult DPs captured at a spring migration stopover.
View Article and Find Full Text PDFInvasive Infections in Children in Vaccine Era: Phenotypic and Genotypic Characterization Tunis, Tunisia.
Microorganisms
December 2024
Laboratory of Microbiology, Children's Hospital of Tunis, Beb Saadoun, Tunis 1007, Tunisia.
The changing epidemiological profile of invasive infections (IIHi) is noted in the post-vaccination era. The aim of this study was to characterize phenotypically and genotypically invasive (Hi) isolates detected in Tunisian pediatric patients. A retrospective study was conducted in the microbiology laboratory of the Children's Hospital of Tunis over ten years (2013-2023).
View Article and Find Full Text PDFEpidemiological and genetic characterization of the influenza A (H1N1) virus in Hangzhou City in 2023.
Front Public Health
December 2024
Department of Clinical Research, The 903rd Hospital of PLA, Hangzhou, China.
Article Synopsis
- The study aimed to investigate the epidemiological and genetic characteristics of the H1N1 influenza virus in Hangzhou City using samples from a hospital between January and March 2023.
- Researchers collected 2,376 respiratory samples, identifying 680 cases of influenza A, from which 112 H1N1 and 17 H3N2 subtypes were isolated for genetic analysis.
- Analysis of HA and NA genes showed high similarity to WHO-recommended vaccine strains, with several amino acid mutations detected in the H1N1 strains that could affect their antigenic properties.
Clinical epidemiology and impact of Haemophilus influenzae airway infections in adults with cystic fibrosis.
BMC Infect Dis
October 2024
Department of Medicine, Cumming School of Medicine, University of Calgary and Alberta Health Services, Calgary, Canada.
Background: Haemophilus influenzae is prevalent within the airways of persons with cystic fibrosis (pwCF). H. influenzae is often associated with pulmonary exacerbations (PEx) in pediatric cohorts, but in adults, studies have yielded conflicting reports around the impact(s) on clinical outcomes such as lung function decline.
View Article and Find Full Text PDFErratum: Development and implementation of a Core Genome Multilocus Sequence Typing (cgMLST) scheme for .
Microb Genom
October 2024
Department of Biology, University of Oxford, UK.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!