AI Article Synopsis
- The study analyzed immune cells from multiple sclerosis (MS) patients using microarray technology, revealing increased immune activation and specific gene expression changes linked to MS.
- Mice lacking the E2f1 gene showed only mild symptoms in a model designed to mimic MS, suggesting E2F's role in disease severity.
- Additionally, Avonex-treated MS patients exhibited lower levels of E2F-related gene expression, implicating these pathways in MS and confirming findings by correlating gene changes to known MS risk factors.
Article Abstract
We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.
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| http://dx.doi.org/10.1016/j.jneuroim.2004.01.017 | DOI Listing |
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