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Analytical application of the reactions of amitriptyline with eriochrome cyanine R and pyrocatechol violet. | LitMetric
Eriochrome cyanine R (ECR) and pyrocatechol violet (PCV) have been tested as reagent for the determination of amitriptyline (AMT). They react in aqueous media with AMT forming coloured compounds sparingly soluble in walter. Optimal conditions for the reaction have been established and new extractive-spectrophotometric methods have been developed for the determination of amitriptyline. It can be assayed in the concentration range 8.0-80.0 microg/ml for the ECR method and in the range 1.5-15.0 microg/ml in the case of PCV. The methods were applied to the determination of amitriptyline in albumin and pharmaceutical preparations. The UV-VIS studies of the reagents and the formed compounds were performed.
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