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Optimization of a rapid, sensitive, and high throughput molecular sensor to measure canola protoplast respiratory metabolism as a means of screening nanomaterial cytotoxicity.
Plant Methods
October 2024
Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada.
Nanomaterial-mediated plant genetic engineering holds promise for developing new crop cultivars but can be hindered by nanomaterial toxicity to protoplasts. We present a fast, high-throughput method for assessing protoplast viability using resazurin, a non-toxic dye converted to highly fluorescent resorufin during respiration. Protoplasts isolated from hypocotyl canola (Brassica napus L.
View Article and Find Full Text PDFImpact of Cavity Length Non-uniformity on Reaction Rate Extraction in Strong Coupling Experiments.
J Am Chem Soc
November 2024
National Academies of Science NRC Post-Doctoral Researcher, Naval Research Laboratory, Chemistry Division, 4555 Overlook Ave SW, Washington, District of Columbia 20375, United States.
Reports of altered chemical phenomena under vibrational strong coupling, including reaction rates, product distributions, intermolecular forces, and cavity-mediated vibrational energy transfer, have been met with a great deal of skepticism due to several irreproducible results and the lack of an accepted theoretical framework. In this work, we add some insight by identifying a UV-vis measurement artifact that distorts observed absorption peak positions, amplitudes, and consequently, chemical reaction rates extracted in optical microcavities. We predict and characterize the behavior of this artifact using the Transfer Matrix (TM) method and confirm its presence experimentally.
View Article and Find Full Text PDFAtacama Large Aperture Submillimeter Telescope (AtLAST) science: Surveying the distant Universe.
Open Res Eur
June 2024
Institute of Theoretical Astrophysics, University of Oslo, Oslo, N-0315, Norway.
During the most active period of star formation in galaxies, which occurs in the redshift range 1 3, strong bursts of star formation result in significant quantities of dust, which obscures new stars being formed as their UV/optical light is absorbed and then re-emitted in the infrared, which redshifts into the mm/sub-mm bands for these early times. To get a complete picture of the high- galaxy population, we need to survey a large patch of the sky in the sub-mm with sufficient angular resolution to resolve all galaxies, but we also need the depth to fully sample their cosmic evolution, and therefore obtain their redshifts using direct mm spectroscopy with a very wide frequency coverage. This requires a large single-dish sub-mm telescope with fast mapping speeds at high sensitivity and angular resolution, a large bandwidth with good spectral resolution and multiplex spectroscopic capabilities.
View Article and Find Full Text PDFNano-Liposomal Beetroot Phyto-Pigment in Photodynamic Therapy as a Prospective Green Approach for Cancer Management: In Vitro Evaluation and Molecular Dynamic Simulation.
Pharmaceutics
August 2024
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
Using plant extracts as photosensitizers in photodynamic therapy (PDT) represents a significant green approach toward sustainability. This study investigates beetroot juice (BRJ), betanin, and their liposomal formulations (Lip-BRJ, Lip-Bet) as photosensitizers in cancer PDT. BRJ was prepared, and its betanin content was quantified via HPLC.
View Article and Find Full Text PDFAdvances in active targeting of ligand-directed polymeric nanomicelles exploiting overexpressed cellular receptors for precise nanomedicine.
RSC Adv
July 2024
Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University Alexandria 21526 Egypt
Many of the utilized drugs which already exist in the pharmaceutical sector are hydrophobic in nature. These drugs are characterized by being poorly absorbed and difficult to formulate in aqueous environments with low bioavailability, which could result in consuming high and frequent doses in order to fulfil the required therapeutic effect. As a result, there is a decisive demand to find modern alternatives to overcome all these drawbacks.
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