AI Article Synopsis
- The Y-box binding protein-1 (YB-1) is important for various biological functions such as controlling gene expression, repairing DNA, and influencing drug resistance.
- Disrupting one allele of the YB-1 gene in mouse embryonic stem cells reduced both mRNA and protein levels by about 50-60%, but did not affect cell growth.
- YB-1(+/-) cells were more sensitive to certain drugs like cisplatin and mitomycin C, suggesting that YB-1 may help protect against DNA damage and contribute to drug resistance.
Article Abstract
The eukaryotic Y-box binding protein-1 (YB-1) functions in various biological processes, including transcriptional and translational control, DNA repair, drug resistance, and cell proliferation. To elucidate the physiological role of the YB-1 protein, we disrupted one allele of mouse YB-1 in embryonic stem (ES) cells. Northern blot analysis revealed that YB-1(+/-) ES cells with one intact allele contain approximately one-half the amount of mRNA detected in wild-type (YB-1(+/+)) cells. We further found that the protein level of YB-1(+/-) cells was reduced to approximately 50-60% compared with that of YB-1(+/+) cells. However, no apparent growth difference was found between YB-1(+/-) and YB-1(+/+) cells. YB-1(+/-) cells showed increased sensitivity to cisplatin and mitomycin C, but not to etoposide, X-ray or UV irradiation, as compared to YB-1(+/+) cells. YB-1 may have the capacity to exert a protective role against cytotoxic effects of DNA damaging agents, and may be involved in certain aspects of drug resistance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11159763 | PMC |
| http://dx.doi.org/10.1111/j.1349-7006.2004.tb03214.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!