Objective: To investigate the effect of cisplatin administration on the ultrastructural morphology of the organ of Corti, stria vascularis and spiral ganglion.

Material And Methods: Forty-eight guinea pigs were treated with cisplatin by daily i.p. injection at a dose of 1.5 mg/kg for eight consecutive days. Electrocochleography was performed at various survival times after the final application of cisplatin. The cochleae were subsequently examined using electron microscopy.

Results: Ultrastructural examination corroborated that, in cochlear turns showing complete loss of outer hair cells (OHCs) at the light microscopic level, OHCs were indeed missing and had been completely replaced by supporting cells. OHC loss, the number of affected OHCs and the degree of intracellular pathology in the OHCs in the 1-day, 1-week and 2-week survival groups were considerably higher than in the 4- and 8-week survival groups. All degenerated OHCs demonstrated ultrastructural features commonly associated with necrosis. No morphological signs of apoptosis were observed. Strial changes consisted of protrusion of the apical membrane of the marginal cells into the scala media, without any other histopathological changes. Intermediate-cell atrophy, apparent as translucent areas at the light microscopic level, consisted of an increase in intercellular space due to shrinkage of intermediate and marginal cells ultrastructurally. Ultrastructural examination of the spiral ganglion showed that vacuolation of the spiral ganglion cells, seen at the light microscopic level, was due to severe swelling of the mitochondria.

Conclusion: The present results corroborate our previous light microscopic findings. However, the ultrastructural results do not allow a conclusion to be drawn concerning whether the observed recovery is due to the formation of new OHCs or to (self-)repair of damaged OHCs, although the latter is less likely.

Download full-text PDF

Source
http://dx.doi.org/10.1080/00016480310015164DOI Listing

Publication Analysis

Top Keywords

light microscopic
16
microscopic level
12
survival times
8
cisplatin administration
8
ultrastructural examination
8
survival groups
8
marginal cells
8
spiral ganglion
8
ohcs
7
ultrastructural
6

Similar Publications

Di-2-(ethylhexyl)phthalate (DEHP) is a phthalate derivative used extensively in a wide range of materials, such as medical devices, toys, cosmetics, and personal care products. Many mechanisms, including epigenetics, may be involved in the effects of phthalates on brain development. In this study, Sprague-Dawley male rats were obtained 21-23 days after their birth (post-weaning) and were exposed to DEHP during the prepubertal period with low-dose DEHP (DEHP-L, 30 mg/kg/day) and high-dose DEHP (DEHP-H, 60 mg/kg/day, 37 days) until the end of adolescence (PND 60).

View Article and Find Full Text PDF

Background And Aims: Probe-based confocal endomicroscopy (pCLE) allows real-time microscopic visualization of the intestinal mucosa surface layers. Despite remission achieved through anti-tumor necrosis factor or vedolizumab therapy, anomalies in the intestinal epithelial barrier are observed in inflammatory bowel disease (IBD) patients. Our study aimed to assess these abnormalities in non-IBD individuals and compare them with IBD patients in endoscopic remission to identify the associated factors.

View Article and Find Full Text PDF

Photopyroelectric tweezers for versatile manipulation.

Innovation (Camb)

January 2025

Center for Intelligent Biomedical Materials and Devices (IBMD), Shenzhen Institute of Advanced Technology (SIAT), Chinese Academy of Sciences (CAS), Shenzhen 518055, China.

Optical tweezers and related techniques offer extraordinary opportunities for research and applications in physical, biological, and medical fields. However, certain critical requirements, such as high-intensity laser beams, sophisticated electrode designs, additional electric sources, or low-conductive media, significantly impede their flexibility and adaptability, thus hindering their practical applications. Here, we report innovative photopyroelectric tweezers (PPT) that combine the advantages of light and electric field by utilizing a rationally designed photopyroelectric substrate with efficient and durable photo-induced surface charge-generation capability, enabling diverse manipulation in various working scenarios.

View Article and Find Full Text PDF

Cdc42 is crucial for the early regulation of hepatic stellate cell activation.

Am J Physiol Cell Physiol

January 2025

Department of Anatomy and Regenerative Biology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.

The activation of hepatic stellate cells (HSCs) from a quiescent state is a cause of liver fibrosis and a therapeutic target. HSCs are resident mesenchymal cells located in the space of Disse, exhibiting specialized morphological characteristics such as a stellate shape, large lipid droplets, and direct adhesions to hepatocytes via microprojections called HSC spines. Morphological alterations in HSCs play a crucial role in initiating their activation.

View Article and Find Full Text PDF

Purpose: To evaluate the shear bond strength (SBS) of stereolithography (SLA), digital light processing (DLP) manufactured, and computer numerical control (CNC) milled zirconia to veneering ceramic.

Materials And Methods: Rectangular shaped zirconia substrates (10 × 5 × 5 mm) were manufactured through SLA, DLP, and CNC technology separately. Their surface roughness was measured and the surface topography was analyzed by atomic force microscope (AFM).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!