AI Article Synopsis
- The study aimed to isolate the gene responsible for high activity beta-galactosidase from Lactobacillus bulgaricus and analyze factors affecting its amplification.
- Researchers used lysozyme treatment and freeze-thaw cycles to extract DNA and explored various template concentrations and annealing temperatures for amplification.
- The optimal conditions identified were a template concentration of 60 ng/microliter and an annealing temperature of 66 degrees C, setting the stage for future research on this gene.
Article Abstract
Objective: To obtain the gene which encode high activity beta-galactosidase from Lactobacillus bulgaricus and study the influencing factors of amplification.
Methods: Lysozyme and freeze-thaw cycles were applied to obtain Lactobacillus bulgaricus DNA; different template concentration and annealing temperature were selected for the amplification.
Results: 60 ng/microliter template concentration and 66 degrees C annealing temperature were the best reaction conditions of amplifying the gene.
Conclusion: This study optimized the template concentration and anneal temperature conditions of amplifying Lactobacillus bulgaricus beta-galactosidase gene, thus providing a foundation for further studies.
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