AI Article Synopsis
- The study aims to explore how isorhamnetin affects HeLa cells by inhibiting growth and inducing apoptosis while also examining its influence on telomerase activity.
- The researchers treated HeLa cells with various concentrations of isorhamnetin for two days, using methods like MTT and Flow-Cytometry to analyze cell growth and telomerase activity.
- Results showed that isorhamnetin significantly inhibited HeLa cell growth and triggered a 31.7% apoptosis rate at a concentration of 20 micrograms/ml, while also reducing telomerase activity.
Article Abstract
Objective: To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells.
Methods: The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity.
Results: The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase.
Conclusion: By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.
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