[The effects of fluoride and dexamethasone on the phenotypic development of osteoblast grown on the demineralized dental root surface].

OBJECTIVE: To investigate the effects of the phenotypic development of osteoblast growing on the periodontitis affected root surface in the condition with fluoride and dexamethasone.METHODS:Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surf ace of cementum treated with various methods.RESULTS:The present study showed that osteoblast treated with fluoride and dexamethasone had a better cellular growth behavior.CONCLUSION: The results demonstrated that demineralization procedure by citric acid might promote the osteoblasts attachment and growth on root surface; and dexamethasone and low dose fluoride were of benefit to maintain the phenotype of osteoblast as well as promote the maturation of bone-like matrix and increase the mineralization formation.