A multilocus restriction typing (MLRT) method was developed to reduce the number of sequencing reactions required to determine the clonal relationships among serogroup B meningococci causing an epidemic in New Zealand. MLRT was a rapid, simple, and inexpensive method, and the results had an excellent correlation with multilocus sequence typing results.
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http://dx.doi.org/10.1128/JCM.42.4.1742-1745.2004 | DOI Listing |
Front Microbiol
December 2024
College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, China.
Introduction: Bunge is an important medicinal legume species widely cultivated in northeastern China (NEC) and northwestern China (NWC) and can establish a symbiotic relationship with nitrogen-fixing rhizobial strains. However, there are limited reports comparing the genetic diversity, differentiation, and gene flow of rhizobial strains associated with this plant in different geographic regions.
Methods: We used multilocus sequence analysis (MLSA) to investigate the phylogeny and genetic diversity of rhizobia and to estimate their intra- and inter-regional gene flow and genetic differentiation based on the analysis of concatenated core genes (, , and ) and the critical symbiotic gene .
PLoS Negl Trop Dis
December 2024
Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America.
Previous studies have reported high diversity between and within populations of Toxoplasma gondii in South America. In the present study, isolates of T. gondii from chickens were obtained from the Amazon region.
View Article and Find Full Text PDFMicrobiol Spectr
December 2024
Department of Nosocomial Infection Administration, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
The clustered regularly interspaced short palindromic repeats (CRISPR)‒CRISPR-associated protein (Cas) and restriction‒modification (R-M) systems are important immune systems in bacteria. Information about the distributions of these two systems in from different hosts and their mutual effect on antibiotic resistance and virulence is still limited. In this study, the whole genomes of 520 strains of from GenBank, including 325 from humans and 195 from animals, were collected for CRISPR‒Cas systems and type I R-M systems, virulence genes, antibiotic resistance genes, and multilocus sequence typing detection.
View Article and Find Full Text PDFMicrob Genom
December 2024
Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Front Plant Sci
November 2024
Soybean Research Institute & MARA National Center for Soybean Improvement & MARA Key Laboratory of Biology and Genetic Improvement of Soybean & State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization & State Innovation Platform for Integrated Production and Education in Soybean Bio-Breeding & Zhongshan Biological Breeding Laboratory & Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China.
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