AI Article Synopsis
- The study aimed to define the immune profile of colon cancer cells, specifically the HR8348 cell line.
- Researchers used 40 different monoclonal antibodies and flow cytometry to analyze the expression of various immune markers on these cells.
- The results showed that while HR8348 lacked several common immune markers, it did express some specific ones, indicating shared characteristics with certain types of white blood cells.
Article Abstract
Objective: To define the immune phenotype of colon cancer cells.
Methods: Using a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.
Results: HR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.
Conclusion: The results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.
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| http://dx.doi.org/10.3785/j.issn.1008-9292.2004.02.006 | DOI Listing |
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