An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC383076PMC
http://dx.doi.org/10.1128/AEM.70.4.2514-2519.2004DOI Listing

Publication Analysis

Top Keywords

growth sporulation
8
sporulation bacillus
8
bacillus cereus
8
cereus atcc
8
atcc 14579
8
defined conditions
8
14579 defined
4
conditions temporal
4
temporal expression
4
expression genes
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!