We report a duplex real-time PCR-based assay for the simultaneous quantitative detection of Listeria spp. and the food-borne pathogen Listeria monocytogenes. The targets of this single tube reaction were the 23S rDNA and hly genes of Listeria spp. and L. monocytogenes, respectively. Our assay was efficient, 100% selective (i.e., it allowed accurate simultaneous identification of 52 L. monocytogenes and 120 Listeria spp. strains through the FAM-labelled hly and the VIC-labelled 23S rDNA probes, respectively); and had a detection limit of one target molecule in 100% (23S rDNA) and 56% (hly) of the reactions. Simultaneous quantification was possible along a 5-log dynamic range, with an upper limit of 30 target molecules and R2 values > 0.995 in both cases. Our results indicate that this assay based on the amplification of the 23S rDNA gene can accurately quantify any mixture of Listeria species and simultaneously unambiguously quantify L. monocytogenes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.femsle.2004.02.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impact of Improved Sanitation Standard Operating Procedures on Microbial Populations at Three Tree Fruit Packing Facilities.
J Food Prot
December 2024
Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA; One Health Microbiome Center, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address:
Cleaning and sanitizing are of vital importance to control Listeria monocytogenes in food processing facilities. Here, we evaluated the effect of four cleaning and sanitation standard operating procedures (SSOPs; T1, T2, T3, T4) on the reduction of total aerobic mesophilic microorganisms, the occurrence of L. monocytogenes, and the microbiota composition in three tree fruit packing facilities (F1, F2, and F3) over two packing seasons (Y1 and Y2).
View Article and Find Full Text PDFPrevalence of Listeria monocytogenes and Listeria species and associated risk factors for contamination of milk and cottage cheese along the value chains in Ethiopia.
Int J Food Microbiol
December 2024
Center for Food Science and Nutrition, Natural and Computational Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia. Electronic address:
This study aimed to comprehensively examine the prevalence of L. monocytogenes and Listeria spp. in dairy products including raw milk, pasteurized milk, and cottage cheese, and identify potential risk factors for contamination throughout the dairy value chain in major milk sheds in Ethiopia.
View Article and Find Full Text PDFInvestigating the properties and antibacterial, antioxidant, and cytotoxicity activity of postbiotics derived from Lacticaseibacillus casei on various gastrointestinal pathogens in vitro and in food models.
GMS Hyg Infect Control
November 2024
Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Background: Postbiotics comprise soluble compounds freed from the structure of destroyed bacteria or created by living bacteria. Such byproducts provide the host with enhanced biological function as well as specific physiological consequences. This research aims to examine the characteristics and possible health advantages of -derived postbiotics.
View Article and Find Full Text PDFWhole-genome sequencing and metagenomics reveal diversity and prevalence of spp. from soil in the Nantahala National Forest.
Microbiol Spectr
December 2024
Department of Food Science, The University of Tennessee, Knoxville, Tennessee, USA.
Article Synopsis
- The study focuses on understanding the distribution and diversity of environmental bacteria linked to foodborne illnesses by examining soil samples from Nantahala National Forest in North Carolina, where different strains were cultured and sequenced.
- It was found that most strains were isolated from lower altitudes, and the research highlighted a trend of decreasing bacterial diversity and abundance of these bacteria as altitude increased.
- Combining traditional culturing techniques with modern metagenomic sequencing, the study enhances our understanding of how these pathogens are distributed in nature and proposes new methods for tracking foodborne contamination sources.
Unlocking Probiotic Potential: Genomic Insights into Weissella paramesenteroides UFTM 2.6.1.
Probiotics Antimicrob Proteins
December 2024
Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil.
Weissella, a genus of lactic acid bacteria, has diverse beneficial attributes including probiotic activity and biotechnological applications. Therefore, the investigation of the Weissella genus has garnered growing interest. In this study, we sequenced the complete genome of Weissella paramesenteroides UFTM 2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!