AI Article Synopsis
- Galectins play a key role in recognizing and differentiating between various glyco-substrates, and this study focuses on CGL2, a fungal galectin, to understand the mechanisms behind this selectivity.
- The crystal structure analysis of CGL2 paired with several substrate complexes reveals that it achieves higher substrate specificity by fitting mammalian blood group A/B type oligosaccharides into a uniquely shaped binding cleft.
- Kinetic experiments with both wild-type and mutant versions of CGL2 indicate that its tetrameric structure is crucial for its function, and the arrangement of its binding sites influences how substrates interact with it.
Article Abstract
Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.str.2004.03.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative study of Idesia polycarpa Maxim cake meal polysaccharides: Conventional versus innovative extraction methods and their impact on structural features, emulsifying, antiglycation, and hypoglycemic properties.
Food Chem
January 2025
College of Food Science and Engineering, Guiyang University, Guiyang, Guizhou 550005, PR China; Engineering Technology Research Center for Processing and Comprehensive Utilization of Idesia polycarpa, National Forestry and Grassland Administration of the People's Republic of China, Guiyang, Guizhou 550005, PR China. Electronic address:
Idesia polycarpa Maxim (IPM) cake meal, a major by-product of oil extraction, is often discarded in large quantities, resulting in considerable waste. This study explored the extraction of IPM polysaccharides (IPMPs) from cake meal using the innovative ultrasonic-assisted three-phase partitioning (UTPP) method, in comparison with conventional techniques, including acid, medium-temperature alkali, chelating agent, and enzyme extraction methods. The IPMP-UT prepared via UTPP method achieved superior extraction efficiency (10.
View Article and Find Full Text PDFUnpacking Performance Factors of Innovation Systems and Studying Germany's Attempt to Foster the Role of the Patient Through a Market Access Pathway for Digital Health Applications (DiGAs): Exploratory Mixed Methods Study.
J Med Internet Res
January 2025
Working Group for Data-Driven Innovation, Hamburg University of Technology, Hamburg, Germany.
Background: Health care innovation faces significant challenges, including system inertia and diverse stakeholders, making regulated market access pathways essential for facilitating the adoption of new technologies. The German Digital Healthcare Act, introduced in 2019, offers a model by enabling digital health applications (DiGAs) to be reimbursed by statutory health insurance, improving market access and patient empowerment. However, the factors influencing the success of these pathways in driving innovation remain unclear.
View Article and Find Full Text PDFDeveloping an Evidence- and Theory-Informed Mother-Daughter mHealth Intervention Prototype Targeting Physical Activity in Preteen Girls of Low Socioeconomic Position: Multiphase Co-Design Study.
JMIR Pediatr Parent
January 2025
School of Public Health, Physiotherapy and Sports Science, University College Dublin, Dublin 4, Ireland.
Background: Preteen girls of lower socioeconomic position are at increased risk of physical inactivity. Parental support, particularly from mothers, is positively correlated with girls' physical activity levels. Consequently, family-based interventions are recognized as a promising approach to improve young people's physical activity.
View Article and Find Full Text PDFHierarchical Self-Assembly of SnO Nanoparticles into Porous Microspheres: Exceptionally Selective Ammonia Sensing at Ambient.
ACS Appl Mater Interfaces
January 2025
Nanomaterials Laboratory, Department of Polymers and Functional Materials, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad 500 007, India.
Herein, porous SnO microspheres in a three-dimensional (3D) hierarchical architecture were successfully synthesized via a facile hydrothermal route utilizing d-(+)-glucose and cetyltrimethylammonium bromide (CTAB), which act as reducing and structure-directing agents, respectively. Controlled adjustment of the CTAB to glucose mole ratio, reaction temperature, reaction time, and the calcination parameters all provided important clues toward optimizing the final morphologies of SnO with exceptional structural stability and reasonable monodispersity. Electron microscopy analysis revealed that microspheres formed were hierarchical self-assemblies of numerous primary SnO nanoparticles of ∼3-8 nm that coalesce together to form nearly monodispersed and ordered spherical structures of sizes in the range of 230-250 nm and are appreciably porous.
View Article and Find Full Text PDFDevelopment and psychometric properties of a quality of life assessment tool for Chinese patients undergoing intravitreal injection (IVI-QoL-23).
Retina
January 2025
Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China.
Purpose: To develop and assess the psychometric properties of a quality of life (QoL) assessment tool for Chinese patients treated with intravitreal injection (IVI) of anti-vascular endothelial growth factor (anti-VEGF).
Methods: We developed a 31-item IVI-QoL questionnaire using semi- structured patient interviews and expert panel consultation, drawing on a study of the literature. After pretesting on a subset of patients undergoing IVI, the questionnaire was pared down to 23 items.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!