AI Article Synopsis
- Carbonyl stress compounds like glyoxal and methylglyoxal are linked to chronic and age-related diseases, prompting the development of a new method for measuring their levels in human urine.
- A high-performance liquid chromatography (HPLC) technique was established to simultaneously quantify these compounds, using a specific fluorescent reaction and sensitive conditions to achieve reliable results after 45 minutes of analysis.
- In healthy individuals, glyoxal levels were found to be 2-3 times higher than methylglyoxal, with both levels interrelated, indicating a potential link to the detoxification process in the body.
Article Abstract
Carbonyl stress compounds such as glyoxal and methylglyoxal have been recently attracting much attention because of their possible clinical significance in chronic and age-related diseases. A high-performance liquid chromatographic procedure has been developed for the simultaneous quantitation of glyoxal and methylglyoxal in human urine. The assay is based on the reaction of these compounds with 1,2-diamino-4,5-dimethoxybenzene to form fluorescent adducts, which are separated by reversed-phase high-performance liquid chromatography in a total run time of 45 minutes and quantitated fluorometrically using 2,3-pentanedione as an internal standard. Derivatization is performed for diluted urine (100-120 mOsm/kg H2O) under acidic conditions (pH 4.5) at 60 degrees C over a prolonged time (15 h) to maximize the yields. The assay is specific and sensitive enough to analyze urinary levels of glyoxal and methylglyoxal with the within- and between-day relative standard deviations of less than 5%. Urinary levels (mean +/- standard deviation, n = 16) of glyoxal and methylglyoxal in healthy subjects were 4.7 +/- 1.35 microg/mg creatinine, 2.2 +/- 0.65 microg/mg creatinine, respectively, the former being 2 to 3 times more than the latter in every subject. The glyoxal and methylglyoxal levels positively correlated with each other, which may suggest that the levels reflect the individual activity of glyoxalase by which both compounds are detoxified.
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| http://dx.doi.org/10.1515/CCLM.2004.027 | DOI Listing |
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