Development of the adaptive response (AR) to the SoxRS-inducers-menadione (O2(-.)-donor), dinitrosyl-iron complex (NO donor) and their simultaneous action was studied in E. coli. Two AR parameters were used: an increasing in viability and decreasing in the soxS gene (SoxRS-regulon) expression in adapted cells. It was shown that namely peroxynitrite (ONOO-), being formed inside the cells from O2-. and NO, was the most cytotoxic agent among the drugs tested. On the one side, an increase in resistance to menadione treatment was selectively demonstrated in adapted E. coli delta oxyR mutant cells, defective in OxyR-regulon activity. On the other side, a decrease in soxS gene expression was marked in the experiments with menadione, as well So, an AR to O2-. superoxide anion was selectively regulated by the SoxRS DNA-repair pathway. OxyR-regulon that is selectively activated by the most redox-cycling agents and controls AR to these agents doesn't provide development of the AR to O2-..
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The expression of different gene constructs in Escherichia coli SM lux biosensor after exposure to drugs.
Sci Rep
December 2024
Department of Agri-Food Engineering and Environmental Management, Bialystok University of Technology, 15-351, Białystok, Poland.
The research used bacterial biosensors containing bacterial luciferase genes to monitor changes in the environment in real-time. In this work to express four different gene constructs: recA:luxCDABE, soxS:luxCDABE, micF:luxCDABE, and rpoB:luxCDABE in Escherichia coli SM lux biosensor after exposure to three different antibiotics (nalidixic acid, ampicillin, kanamycin) and diclofenac was determined. It was found that incubation of the E.
View Article and Find Full Text PDFNatural Inhibitors of MDR Efflux Pumps AcrAB and AcrD: An Integrated In Silico, Molecular, and In Vitro Investigation.
Int J Mol Sci
December 2024
Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
Multidrug-resistant (MDR) remains a significant global health threat. This study aimed to explore the potential of essential oil components as novel inhibitors of the MDR efflux pumps AcrAB and AcrD. isolates were characterized for serotype, antibiotic resistance, and efflux pump activity.
View Article and Find Full Text PDFPhenotypic Profiling of Tigecycline-resistant Klebsiella pneumoniae Strains Induced In vitro.
Curr Microbiol
December 2024
Medical School of Chinese PLA, Beijing, China.
Tigecycline is one of the last-resort treatment options for infections caused by carbapenem-resistant Klebsiella pneumoniae (KP). Unfortunately, tigecycline resistance is increasingly reported and causes an unprecedented public health crisis worldwide. Although studies on tigecycline resistance are expanding, the underlying mechanisms are not fully understood.
View Article and Find Full Text PDFAntibiotic tolerance due to restriction of cAMP-Crp regulation by mannitol, a non-glucose-family PTS carbon source.
mSphere
December 2024
State Key Laboratory of Vaccines for Infectious Diseases, Xiang-An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, Xiamen University, Xiamen, Fujian Province, China.
Article Synopsis
- - The study reveals that Enzyme-IIA (EIIA) links glucose sugar uptake with a regulatory system affecting antibiotic resistance, where phosphorylated EIIA increases cAMP-Crp activity, leading to cell death through reactive oxygen species (ROS) accumulation.
- - Mannitol, unlike other sugars that require EIIA for uptake, has been shown to reduce the effectiveness of antibiotics without changing their minimum inhibitory concentration, indicating it promotes antibiotic tolerance by inhibiting cAMP-Crp action.
- - This research highlights that other non-glucose carbon sources like mannose and sorbitol can also induce similar antibiotic tolerance through a mechanism that lowers ROS activity, posing challenges for effective infection treatment.
Colistin Resistance Mechanisms and Molecular Epidemiology of c Complex Isolated from a Tertiary Hospital in Shandong, China.
Infect Drug Resist
August 2024
Department of Clinical Laboratory, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
Background: complex (ECC), which includes major nosocomial pathogens, causes urinary, respiratory, and bloodstream infections in humans, for which colistin is one of the last-line drugs.
Objective: This study aimed to analyse the epidemiology and resistance mechanisms of colistin-resistant complex (ECC) strains isolated from Shandong, China.
Methods: Two hundred non-repetitive ECC strains were collected from a tertiary hospital in Shandong Province, China, from June 2020 to June 2022.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!