AI Article Synopsis
- The study reexamined the family classification of the spider genus Nephila using molecular phylogenetic analyses of certain gene fragments.
- The findings indicated that Araneidae and Tetragnathidae are not closely related, suggesting that Nephila is more closely related to Araneidae, implying a potential reclassification back to this family.
- Additionally, the research identified the 3'-end non-repetitive region of the MaSp1 gene as useful molecular markers for studying spider phylogeny.
Article Abstract
The family status of the genus Nephila, which belongs to Tetragnathidae currently but Araneidae formerly, was reexamined based on molecular phylogenetic analyses. In the present study, 12S and 18S rRNA gene fragments of eight species of spiders were amplified and sequenced. In addition, 3'-end partial cDNA of major ampullate spidroin-1 (MaSp1) gene of Argiope amoena was cloned and sequenced, and the 3'-end non-repetitive region's cDNA sequence of MaSp1 gene and the predicted amino acid sequence of C-terminal non-repetitive region of MaSp1 were aligned with some previously known sequences. The resulting phylogeny showed that Araneidae and Tetragnathidae are not a sister group in the superfamily Araneoidea, and the genus Nephila is closer to the genera of the family Araneidae rather than to those of Tetragnathidae. We suggest that the genus Nephila should be transferred back to Araneidae. Or the subfamily Nephilinae might be elevated to family level after it was redefined and redelimited. Furthermore, the study showed that 3'-end non-repetitive region's cDNA sequence of MaSp1 gene and C-terminal non-repetitive region's amino acid sequence of MaSp1 are useful molecular markers for phylogenetic analysis of spiders.
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| http://dx.doi.org/10.2108/zsj.21.343 | DOI Listing |
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