Plastic wastes constitute a worldwide environmental problem, and the demand for biodegradable plastics has become high. One of the most important characteristics of microbial polyesters is that they are thermoplastic with environmentally degradable properties. In this study, pUC19/PHA was cloned and transformed into three different Escherichia coli strains. Among the three strains that were successfully expressed in the production of polyhydroxyalkanoates (PHA), E. coli HMS174 had the highest yield in the production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[HB-HV]). The cell dry weight and PHA content of recombinant HMS174 reached as high as 10.27 g/L and 43% (w/w), respectively, in fed-batch fermentor culture. The copolymer of PHA, P(HB-HV), was found in the cells, and the biopolymers accumulated were identified and analyzed by gas chromatography, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry. We demonstrated clearly that the E. coli host for PHA production has to be carefully selected to obtain a high yield. The results obtained indicated that a superior E. coli with high PHA production can be constructed with a desirable ratio of P(HB-HV), which has potential applications in industry and medicine.
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Microbiol Spectr
January 2025
National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health, Tel Aviv, Israel.
Unlabelled: Carbapenem-resistant Enterobacterales (CRE) are divided into two distinct groups: carbapenemase-producing (CPE) and non-carbapenemase-producing (non-CPE). The population of non-CPE growing on CPE selective plates during routine screening is usually not reported and is not well defined. This study aimed to characterize non-CPE isolates growing on those plates.
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Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Bacterial infections can induce exuberant immune responses that can damage host tissues. Previously, we demonstrated that systemic infection in mice causes tissue damage in the liver. This liver necrosis is associated with the expression of endogenous retroviruses, chromosomally integrated retroviruses that encode a reverse transcriptase.
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Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
One of the main difficulties in nanotechnology is the development of an environmentally friendly, successful method of producing nanoparticles from biological sources. Silver-doped zinc oxide nanoparticles (Ag-ZnO NPs), with antibacterial and antioxidant properties, were produced using Adiantum venustum extract as a green technique. Fresh A.
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January 2025
Department of Environmental Sciences, Southern Illinois University Edwardsville, 44 Circle Drive SW 2145, PO Box 1099, Edwardsville, IL, USA, 62026.
The designated uses of lakes connect individuals to the natural environment, but some can expose recreational users to pathogens associated with fecal contamination that cause waterborne illnesses. Routine monitoring of fecal indicators in surface waters helps identify and track sources of fecal contamination to protect public health. We examined fecal indicators ( and enterococci) and factors influencing recreational freshwater quality.
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January 2025
Department of Food Sensory and Cognitive Science, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.
The rapid evolution of nanotechnology has catalyzed significant advancements in the design and application of nano-sensors, particularly within the food industry, where ensuring safety and quality is of paramount concern. This review explores the multifaceted role of nano-sensors constructed from diverse nanomaterials in detecting foodborne pathogens and toxins, offering a comprehensive analysis of their operational principles, sensitivity, and specificity. Nano-sensors leverage unique physical and chemical properties at the nanoscale to enhance the detection of microbial contamination, actively contributing to food safety protocols.
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