AI Article Synopsis
- Tryptophan 214 is the only tryptophan in human serum albumin (HSA) and is found in the crucial subdomain 2A ligand binding site.
- The study examined the fluorescence lifetime of tryptophan 214 across various HSA mutants with substitutions in subdomain 2A and found notable changes in lifetimes for those with significant environmental modifications.
- The results indicated that mutations near tryptophan 214, as well as some distant substitutions, affected its fluorescence, showcasing the conformational flexibility of subdomain 2A in HSA.
Article Abstract
Tryptophan 214, the only tryptophan residue in human serum albumin, is located in the physiologically important subdomain 2A ligand binding site. In the present study the fluorescence lifetime of tryptophan 214 in the following human serum albumin (HSA) mutants with substitutions in subdomain 2A were determined: K195M, K199M, F211V, R218M, R218H, R218A, R222M, H242V, and R257M. An HSA mutant in which tryptophan was moved from subdomain 2A to subdomain 3A (W214L/Y411W) was also examined. Additionally, the fluorescence lifetime of tryptophan 214 in an HSA fragment consisting of subdomains 1A, 1B, and 2A (1A-1B-2A HSA) was determined. For those species expected to have the most dramatic changes in tryptophan microenvironment, W214L/Y411W and 1A-1B-2A HSA, clear changes in tryptophan lifetimes were observed. Significant changes were also seen for those species with mutations at position 218, which is next to tryptophan in the X-ray structure of HSA. However, significant changes were also observed for H242V and R257M, which contain substitutions at positions not immediately adjacent to tryptophan 214, highlighting the conformational flexibility of subdomain 2A.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1385/CBB:40:2:115 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association between large neutral amino acids and white matter hyperintensities in middle-aged adults at varying metabolic risk.
Brain Imaging Behav
December 2024
Department of Psychology, The University of Texas at Austin, 108 E. Dean Keeton Street, Stop A8000, Austin, TX, 78712, USA.
This investigation delves into the interplay between large neutral amino acids (LNAA) and metabolic syndrome (MetS) in midlife adults, examining their collective influence on brain structure. While LNAA, such as tryptophan and phenylalanine, are known to bolster cognition in youth, these relationships often reverse later in life. Our study hypothesized an earlier reversal of these benefits in middle-aged adults with MetS, potentially signaling premature brain aging.
View Article and Find Full Text PDFExperimental and computational insights of Albizia amara phytoconstituents targeting anthranilate phosphoribosyltransferase from Malassezia globosa.
Acta Trop
November 2024
Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India. Electronic address:
The fungus Malassezia globosa is often responsible for superficial mycoses posing significant treatment challenges because of the unfavourable side effects of available antifungal drugs. To reduce potential hazards to the host and overcome these hurdles, new therapeutic medicines must be developed that selectively target enzymes unique to the pathogen. This study focuses on the enzyme anthranilate phosphoribosyltransferase (AnPRT), which is vital to M.
View Article and Find Full Text PDFVersatile Bovine Serum Albumin as Ingenious Electron Operator-Enhanced Photoelectrochemical Biosensing for Ultrasensitive Detection of miRNA.
Anal Chem
September 2024
Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, Key Laboratory for Green Chemistry of Jiangxi Province, College of Chemistry and Materials, Jiangxi Normal University, Nanchang 330022, People's Republic of China.
Bovine serum albumin (BSA) has been widely used in biosensors as a blocking agent. Herein, conformist BSA was first exploited as an ingenious operator to enhance the photocurrent response of (2,2')-2,2'-(1,4-phenylene)bis(3-(4-(bis(4-methoxyphenyl)amino)phenyl)acrylonitrile) (TPDCN)-based photoelectrochemical (PEC) platform via manipulating the electron transfer process of the detection system. Concretely, the presence of target molecules triggered catalytic hairpin assembly reaction and subsequently powered terminal deoxynucleotidyl transferase-mediated signal amplification to produce the AgNP@BSA-DNA dendrimer nanostructure.
View Article and Find Full Text PDFExploring the Surface: Sampling of Potential Skin Cancer Biomarkers Kynurenine and Tryptophan, Studied on 3D Melanocyte and Melanoma Models.
Biomolecules
July 2024
Department of Biomedical Science, Faculty of Health and Society, Malmö University, 205 06 Malmo, Sweden.
Early detection of cancer via biomarkers is vital for improving patient survival rates. In the case of skin cancers, low-molecular-weight biomarkers can penetrate the skin barrier, enabling non-invasive sampling at an early stage. This study focuses on detecting tryptophan (Trp) and kynurenine (Kyn) on the surface of reconstructed 3D melanoma and melanocyte models.
View Article and Find Full Text PDFArbuscular mycorrhizal fungi regulate amino acid metabolism, phytohormones and glycolysis pathway to promote the growth of Suaeda salsa under combined Cd and NaCl stresses.
Plant Physiol Biochem
September 2024
Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Collaborative Innovation Center for Grassland Ecological Security Jointly Supported by the Ministry of Education of China and Inner Mongolia Autonomous Region, Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, China. Electronic address:
The use of halophytes in conjunction with arbuscular mycorrhizal (AM) fungi has been found to enhance the removal efficacy of heavy metals and salts in heavy metals contaminated saline soil. The mechanisms of AM fungi on promoting halophyte growth and regulating metabolism remain unclear. In this study, combinations of 0 g kg NaCl and 3 mg kg Cd (S0Cd3), 6 g kg NaCl and 3 mg kg Cd (S6Cd3), and 12 g kg NaCl and 3 mg kg Cd (S12Cd3) were employed to explore the impact of Funneliformis mosseae on the growth and metabolism of Suaeda salsa.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!