AI Article Synopsis
- A panel of 20 porcine sera was distributed for testing across 5 labs in Europe and Canada for porcine circovirus type 2 antibodies.
- Results showed significant variations in titers between labs, influenced by the assay used and type of fixative applied.
- The study emphasizes the necessity for standardized testing procedures and biological materials to ensure consistency in results.
Article Abstract
A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assay (IPMA), and to determine the titer of each serum. Results from all centers were then compiled and correlated. They demonstrate a wide variation in the titers obtained between laboratories. These differences were dependent on the assay used and the choice of fixative. In general, IPMA gave higher titers than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus.
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| http://dx.doi.org/10.1177/104063870401600214 | DOI Listing |
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