By means of culture medium, it was researched the position of the colony of Staphylococcus aureus on the mattress, to evaluate the efficaciousness of the methods of cleaning and disinfection of the river bed in the Faculdade de Medicina do Triângulo Mineiro's School Hospital (Uberaba). It were evaluated fifty mattresses on the period of October 22nd (2000) to January 16th (2001). The samples were collected and grown, the exhaustion technique draining, on two different nutrient bases (blood agar and mannitol salt agar) followed by catalase and coagulase tests. For the statistical analysis, were used non-parametrics tests Mann-Whitney, Kruskal-Wallis, Wilcoxon Matched Pairs Test with significance level p < 0.05 were used. Six hundred dishes of culture medium have been used. There was growing in 94 (15.6%), being 82 (87.2%) before and 12 (12.8%) after cleaning and disinfection. Concerning the position on the bed, the samples obtained from mannitol salt agar medium showed significant retention on the lower position of bed. The results alert to flaws in the procedure for cleaning and disinfection from the mattresses studied by us.

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