AI Article Synopsis
- Agrobacterium tumefaciens transfers DNA to plant cells via a single-stranded DNA molecule linked to the VirD2 protein, which has signals that help it enter the plant nucleus.
- A tomato cDNA clone called DIG3 was identified, encoding a protein that interacts with the C-terminal part of VirD2 and functions as a type 2C serine/threonine protein phosphatase.
- Overexpression of DIG3 in tobacco cells was found to inhibit the nuclear import of a fusion protein containing VirD2, suggesting DIG3's role in facilitating the entry of the VirD2 protein and the DNA it carries into the plant nucleus.
Article Abstract
Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded DNA molecule (the T-strand) covalently linked to VirD2 protein. VirD2 contains nuclear localization signal sequences that presumably help direct the T-strand to the plant nucleus. We identified a tomato cDNA clone, DIG3, that encodes a protein that interacts with the C-terminal region of VirD2. DIG3 encodes an enzymatically active type 2C serine/threonine protein phosphatase. Overexpression of DIG3 in tobacco BY-2 protoplasts inhibited nuclear import of a beta-glucuronidase-VirD2 nuclear localization signal fusion protein. Thus, DIG3 may be involved in nuclear import of the VirD2 protein and, consequently, the VirD2/transferred DNA complex.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC387391 | PMC |
| http://dx.doi.org/10.1073/pnas.0300084101 | DOI Listing |
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