AI Article Synopsis
- Neurotoxin II from cobra venom is a short alpha-neurotoxin that disrupts nicotinic acetylcholine receptors.
- The gene for this toxin was combined with the thioredoxin gene and a specific linker to enable its expression in bacteria, although cyanogen bromide was more effective for cleavage than the intended enzyme.
- The final purified neurotoxin was confirmed to match the native version through various analytical techniques, with a yield of 6 mg per liter of bacterial culture.
Article Abstract
Neurotoxin II from the venom of cobra Naja oxiana is a short type alpha-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.
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