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Serologic and molecular biologic methods for SARS-associated coronavirus infection, Taiwan. | LitMetric

AI Article Synopsis

  • Severe Acute Respiratory Syndrome (SARS) has been a global concern since March 2003, following the identification of its virus, SARS-CoV.
  • This study assessed four serologic tests for detecting antibodies against SARS-CoV in 537 probable SARS patients, comparing their performance to the RT-PCR method.
  • Results showed high sensitivity and specificity for the ELISA and IFA tests, while ICT had lower sensitivity; overall, there was significant agreement among the different testing methods.

Article Abstract

Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase-polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR. With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus-based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322922PMC
http://dx.doi.org/10.3201/eid1002.030731DOI Listing

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