The need for platelet transfusion has increased at the University College Hospital (U.C.H.) Ibadan, Nigeria because of improved patient care and use of intensive therapeutic regimen associated with bone marrow aplasia. Therefore there is a need to assess the quality of the platelet concentrate prepared in our environment with a view to improving the quality of services available, consequently this study was carried out. Fifty platelet concentrates were assessed for pH, swirling index, volume, platelet count, WBC count and red cell count. All the concentrate preparations were within acceptable pH value of 7.25. The average volume was 18.52 mls/bag. The average platelet count per concentrate was 41.7+/-39.5 x 10(9)/L. Thirty-five percent (35%) of the platelet concentrates had a value > 55 x 10(9)/L. White blood cell count (WBC) < 12 x 10(9)/L was seen 49% of the platelet concentrates prepared. Forty percent (40%) of the platelet concentrate had a red blood cell count (RBC count) > 12 x 10(9)/L with 30% not having red cell contamination. Swirling test was positive in 72% of the platelet concentrate units. The results from this study point to the need to improve the quality of the platelet concentrates being prepared in our blood bank in order to get maximum therapeutic values. There is also a need for regular quality control of the platelet concentrate being prepared in our blood bank.
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J Med Biochem
November 2024
General Hospital of Verona, Service of Laboratory Medicine, Verona, Italy.
Background: Low-volume blood tubes offer several advantages in facilitating blood collection, reducing iatrogenic anemia and spurious hemolysis, but their clinical reliability must be validated. We planned this investigation for establishing the reliability of routine hematologic testing in low-volume tubes before their implementation into clinical practice.
Methods: Blood was drawn from 44 ostensibly healthy laboratory professionals into three blood tubes, as follows: 3.
Clin Adv Periodontics
January 2025
Department of Periodontics and Implantology, SRM Dental College and Hospital, Chennai, India.
Background: Newer generation platelet concentrates, such as advanced platelet-rich fibrin plus (A-PRF+) obtained following low-speed centrifugation concept and horizontal platelet-rich fibrin (H-PRF) obtained from swing out and bucket system, showed increased platelet entrapment and growth factor release in the in-vitro studies. This prospective study aimed to evaluate and compare the clinical outcomes of A-PRF+ and H-PRF membranes in the treatment of gingival recession defects. The objectives of this study were to compare the changes in the recession height (RH) and the mean root coverage percentage (MRC%) between and within the research groups.
View Article and Find Full Text PDFInt Arch Otorhinolaryngol
January 2025
Department of Ear, Nose and Throat, JJM Medical College, Davangere, Karnataka, India.
Platelet-rich plasma (PRP) contains high platelet concentration and growth factors that help in rapid wound healing, hemostasis, and decreased scarring. It has been used in various conditions to aid in healing, but its use in ear, nose, and throat (ENT) is not yet common. To compare the outcome of using PRP with myringoplasty with that of myringoplasty alone in the repair of tympanic membrane perforations.
View Article and Find Full Text PDFPLoS One
January 2025
Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Muenster, Muenster, Germany.
Weibel-Palade bodies (WPB) are secretory organelles exclusively found in endothelial cells and among other cargo proteins, contain the hemostatic von-Willebrand factor (VWF). Stimulation of endothelial cells results in exocytosis of WPB and release of their cargo into the vascular lumen, where VWF unfurls into long strings of up to 1000 µm and recruits platelets to sites of vascular injury, thereby mediating a crucial step in the hemostatic response. The function of VWF is strongly correlated to its structure; in order to fulfill its task in the vascular lumen, VWF has to undergo a complex packing/processing after translation into the ER.
View Article and Find Full Text PDFAm J Sports Med
January 2025
Department of Orthopaedic Surgery, Sports Medicine Institute, Hospital for Special Surgery, New York, New York, USA.
Background: Randomized controlled trials (RCTs) evaluating the efficacy of platelet-rich plasma (PRP) for the management of lateral epicondylitis (LE) have been characterized by substantial variability in reported outcomes. The source of this heterogeneity is uncertain.
Purpose: To determine the effect of estimated platelet concentration on the efficacy of PRP for the management of LE.
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