Lung macro- and microvascular endothelial cells exhibit unique functional attributes, including signal transduction and barrier properties. We therefore sought to identify structural and functional features of endothelial cells that discriminate their phenotypes in the fully differentiated lung. Rat lung macro- (PAEC) and microvascular (PMVEC) endothelial cells each exhibited expression of typical markers. Screening for reactivity with nine different lectins revealed that Glycine max and Griffonia (Bandeiraea) simplicifolia preferentially bound microvascular endothelia whereas Helix pomatia preferentially bound macrovascular endothelia. Apposition between the apical plasmalemma and endoplasmic reticulum was closer in PAECs (8 nm) than in PMVECs (87 nm), implicating this coupling distance in the larger store operated calcium entry responses observed in macrovascular cells. PMVECs exhibited a faster growth rate than did PAECs and, once a growth program was initiated by serum, PMVECs sustained growth in the absence of serum. Thus, PAECs and PMVECs differ in their structure and function, even under similar environmental conditions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mvr.2003.11.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Endothelial dysfunction in adults with congenital heart disease: A systematic review and meta-analysis.
Eur J Clin Invest
December 2024
First Department of Cardiology, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Background: Adults with congenital heart disease (ACHD) can face a lifelong risk of premature cardiovascular events. Endothelial dysfunction and arterial stiffness may be some of the key mechanisms involved. Early identification of endothelial damage in ACHD could be crucial to mitigate the adverse events.
View Article and Find Full Text PDFUncertainty-aware automatic TNM staging classification for [F] Fluorodeoxyglucose PET-CT reports for lung cancer utilising transformer-based language models and multi-task learning.
BMC Med Inform Decis Mak
December 2024
School of Biomedical Engineering and Imaging Sciences, King's College London, London, UK.
Background: [F] Fluorodeoxyglucose (FDG) PET-CT is a clinical imaging modality widely used in diagnosing and staging lung cancer. The clinical findings of PET-CT studies are contained within free text reports, which can currently only be categorised by experts manually reading them. Pre-trained transformer-based language models (PLMs) have shown success in extracting complex linguistic features from text.
View Article and Find Full Text PDFUsing Large Language Models for Efficient Cancer Registry Coding in the Real Hospital Setting: A Feasibility Study.
Pac Symp Biocomput
December 2024
Department of Computer Science, National Yang Ming Chiao Tung University Hsinchu, 300093, Taiwan, ROC, Taiwan.
The primary challenge in reporting cancer cases lies in the labor-intensive and time-consuming process of manually reviewing numerous reports. Current methods predominantly rely on rule-based approaches or custom-supervised learning models, which predict diagnostic codes based on a single pathology report per patient. Although these methods show promising evaluation results, their biased outcomes in controlled settings may hinder adaption to real-world reporting workflows.
View Article and Find Full Text PDFArticle Synopsis
- The study presents an optimized workflow for analyzing formalin-fixed, paraffin-embedded (FFPE) patient tissues to uncover molecular insights linked to clinical outcomes, utilizing advanced techniques like Adaptive Focused Acoustics (AFA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- The method allows for the analysis of up to 96 samples, identifying between 8,000-10,000 unique proteins with a high level of quantitative accuracy (<20% median CVs).
- Applied to lung adenocarcinoma FFPE blocks, the workflow demonstrates superior deep proteome coverage and efficiency, significantly contributing to biomarker discovery and proteomic research in archived samples.
Costs of stereotactic ablative radiotherapy compared to conventional radiotherapy in the treatment of non-small cell lung cancer - a micro-costing study using Time-Driven Activity Based Costing (TDABC).
BMC Health Serv Res
November 2024
Departamento de Medicina Preventiva, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Av Dr Arnaldo 455, CEP: 01246903, Sao Paulo, SP, Brazil.
Background: Lung cancer is one of the leading causes of morbidity and mortality in Brazil. Radiotherapy is an important therapeutic option, but the techniques used remain subjects of discussion. In this study, we compared the costs of conventional radiotherapy (CRT) and stereotactic ablative radiotherapy (SABR) in the treatment of early-stage non-small cell lung cancer (NSCLC).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!