AI Article Synopsis
- Pseudomonas syringae showed different biochemical responses when cultured with aluminium, chromium, and manganese in a defined citrate medium.
- Aluminium ended up mainly as an insoluble residue, with little detected in the supernatant or bacterial cells.
- In contrast, chromium was partially secreted into the supernatant and very little was taken up by the cells, while manganese was mostly biotransformed into an insoluble pellet.
Article Abstract
Pseudomonas syringae cultured in a defined citrate medium supplemented with 1 mM aluminium, chromium and manganese, respectively, appeared to elicit disparate biochemical responses. At the stationary phase of growth aluminium was predominantly present as an insoluble residue. Although virtually none of this metallic element was detected in the supernatant, the bacterial cells appeared to contain some aluminium. Following the initial uptake of chromium the microbe secreted the metal in the supernatant. Only a small fraction of the chromium was localised in the bacterial cells; 91% manganese was biotransformed into an insoluble pellet. No citrate was detected in the exocellular fluid at cessation of cellular growth.
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