Objective: To establish a method for HPLC fingerprint determination of the alkaloids in S. flavescens.
Method: RP-HPLC, linear gradient elution, LC/MS, etc. were used to determine the fingerprint and identify the main peaks in the HPLC fingerprint.
Result: A satisfactory method for HPLC fingerprint determination of the alkaloids in S. flavescens. was established, and 5 peaks in the HPLC fingerprint were identified.
Conclusion: The perfect fingerprint can be obtained and the method can be used for quality control of S. flavescens.
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