The separation of (86)Y from (86)Sr was optimized by a semi-automated purification system involving the passage of the target sample through three sequential columns. The target material was dissolved in 4 N HNO(3) and loaded onto a Sr-selective (Sr-Spec) column to retain the (86)Sr. The yttrium was eluted with 4 N HNO(3) onto the second Y-selective (RE-Spec) column with quantitative retention. The RE-Spec column was eluted with a stepwise decreasing concentration of HNO(3) to wash out potential metallic impurities to a waste container. The eluate was then pumped onto an Aminex A5 column with 0.1 N HCl and finally with 3 N HCl to collect the radioyttrium in 0.6-0.8 mL with a >80% recovery. This method enabled us to decontaminate Sr by 250,000 times and label 30 micro g of DOTA-Biotin with a >95% yield.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.nucmedbio.2003.07.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Combined cation-exchange and extraction chromatography for the concomitant separation of Zr, Hf, Th, and the Lanthanides from geological materials.
Talanta
May 2002
Département de Géologie, O.P.G.C., C.N.R.S., Université Blaise Pascal, Centre Régional de Mesures Physiques 5, rue Kessler, 63038 Clermont-Ferrand, Cedex, France.
A new method was developed, aiming to overcome the iron and titanium interferences which severely limit the applications of a recently introduced extraction chromatography material, referred to as RE resin (formerly, RE Spec), to the analysis of geological samples. The separation scheme is based on the combined use, in tandem columns, of the conventional AG50WX4 cation-exchange resin and the RE extraction chromatography material, without any intervening evaporation step. The Lanthanides are recovered quantitatively, while recoveries for Zr, Hf and Th range from 94 to 98%.
View Article and Find Full Text PDFSemi-automated 86Y purification using a three-column system.
Nucl Med Biol
February 2004
Nuclear Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
The separation of (86)Y from (86)Sr was optimized by a semi-automated purification system involving the passage of the target sample through three sequential columns. The target material was dissolved in 4 N HNO(3) and loaded onto a Sr-selective (Sr-Spec) column to retain the (86)Sr. The yttrium was eluted with 4 N HNO(3) onto the second Y-selective (RE-Spec) column with quantitative retention.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!