An isocratic high-performance liquid chromatographic method for the quantitation of felbamate in serum is presented. The method is based on protein precipitation with acetonitrile and reversed-phase chromatography with spectrophotometric detection at 210 nm. The separation was performed on a Nova-Pak C18 column and the mobile phase consisted of acetonitrile-water (1:4, v/v). Phenobarbital was applied as an internal standard. The assay was linear over the concentration range 0.5-200.0 microg/ml (r = 0.9999). Intra-day and inter-day precision expressed by relative standard deviation is less than 3%. The percentage of recovery was 98.63 +/- 2.47. The method is suitable for drug level monitoring and for pharmacokinetic studies.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
An accessible workflow for high-sensitivity proteomics using parallel accumulation-serial fragmentation (PASEF).
Nat Protoc
January 2025
Department Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
Deep and accurate proteome analysis is crucial for understanding cellular processes and disease mechanisms; however, it is challenging to implement in routine settings. In this protocol, we combine a robust chromatographic platform with a high-performance mass spectrometric setup to enable routine yet in-depth proteome coverage for a broad community. This entails tip-based sample preparation and pre-formed gradients (Evosep One) combined with a trapped ion mobility time-of-flight mass spectrometer (timsTOF, Bruker).
View Article and Find Full Text PDFLiquid Metal Nanobiohybrids for High-Performance Solar-Driven Methanogenesis via Multi-Interface Engineering.
Angew Chem Int Ed Engl
January 2025
Fujian Agriculture and Forestry University, College of Resources and Environment, CHINA.
Nanobiohybrids for solar-driven methanogenesis present a promising solution to the global energy crisis. However, conventional semiconductor-based nanobiohybrids face challenges such as limited tunability and poor biocompatibility, leading to undesirable spontaneous electron and proton transfer that compromise their structural stability and CH4 selectivity. Herein, we introduced eutectic gallium-indium alloys (EGaIn), featuring a self-limiting surface oxide layer surrounding the liquid metal core after sonication, integrated with Methanosarcina barkeri (M.
View Article and Find Full Text PDFLiquid-infused nanostructured composite as a high-performance thermal interface material for effective cooling.
Nat Commun
January 2025
Department of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA, USA.
Effective heat dissipation remains a grand challenge for energy-dense devices and systems. As heterogeneous integration becomes increasingly inevitable in electronics, thermal resistance at interfaces has emerged as a critical bottleneck for thermal management. However, existing thermal interface solutions are constrained by either high thermal resistance or poor reliability.
View Article and Find Full Text PDFTuning Dual Catalytic Active Sites of Pt Single Atoms Paired with High-Entropy Alloy Nanoparticles for Advanced Li-O Batteries.
ACS Nano
January 2025
Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, School of Materials Science and Engineering, Shandong University, Jinan 250061, P. R. China.
To achieve a long cycle life and high-capacity performance for Li-O batteries, it is critical to rationally modulate the formation and decomposition pathway of the discharge product LiO. Herein, we designed a highly efficient catalyst containing dual catalytic active sites of Pt single atoms (Pt) paired with high-entropy alloy (HEA) nanoparticles for oxygen reduction reaction (ORR) in Li-O batteries. HEA is designed with a moderate d-band center to enhance the surface adsorbed LiO intermediate (LiO(ads)), while Pt active sites exhibit weak adsorption energy and promote the soluble LiO pathway (LiO(sol)).
View Article and Find Full Text PDFAssociation of Organophosphorus Pesticide and Glyphosate Exposure with Nasal Flora and Total IgE in Solar Greenhouse Workers: A Unique Farmer Group.
Environ Pollut
January 2025
Beijing Institute of Occupational Disease Prevention and Control, Beijing 100093, China. Electronic address:
Studies have shown that the presence of allergens, including insecticides, significantly increases the risk of occupational allergic diseases among solar greenhouse workers. However, no studies have yet investigated the relationship between organophosphorus pesticide use by greenhouse workers and allergic diseases, and the role of the flora in this context remains unclear. Therefore, this study aimed to investigate the relationship between combined exposure to organophosphorus pesticides (OPs) and Glyphosate (GLY) and changes in total immunoglobulin E (IgE) levels, as well as to analyze the role of nasal flora in allergic status.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!