AI Article Synopsis

  • Objective: Develop a long-term in vitro culture system for human neural stem cells (hNSCs) to investigate their biological properties.
  • Methods: Used a serum-free medium with growth factors to continuously culture hNSCs from 20-week-old human fetal tissue and analyze their growth, cell cycle, and differentiation through flow cytometry.
  • Results: hNSCs maintained consistent exponential growth and multi-lineage differentiation potential over ten months of culture, preserving their biological characteristics despite long-term passaging.

Article Abstract

Objective: To set up long-term in vitro culture system of the human neural stem cells (hNSC) and to study their biological properties.

Methods: Human fetuses aged about 20 weeks following spontaneous abortion were adopted. A serum-free medium containing basic fibroblast growth factor and epidermal growth factor was used to make the hNSCs divide continuously in the culture. The growth curve of continually passaged cells was examined. The effects of long-term culture on the cell cycle, cell differentiation were analyzed. The cell cycles of these cells were analyzed using flow cytometry.

Results: The cells from the human embryonic cortical tissue could be maintained and propagated in the presence of growth factors. Neurospheres were generated continually. Only one month after the primary culture, the precursors could be effectively discarded. The cells could be cultured for ten months. The cells had an exponential, consistent growth. The cell cycle analysis indicated that the hNSCs maintained remarkable proliferation. Upon differentiation, the hNSCs gave rise to mature cells. The multi-lineage potential of differentiation after different passages remained unchanged.

Conclusion: The hNSCs isolated from the human embryonic tissues retained their biological features after long-term culture in vitro.

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Source
http://dx.doi.org/10.1111/j.1749-0774.2003.tb00147.xDOI Listing

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