[The fibroblast primary cell culture by the split-thickness skin slide technique].

Objective: To acquire lots of cell to culture during the primary cell culture.

Method: We take the split-thickness skin slide technique to acquire the dissociated fibroblast cell in two big-ear rats.

Results: The cell number is above 10(6) from 1 cm x 2 cm split-thickness skin slide and the technique is simple, economic, effectve.

Conclusion: We think this way is better than other methods, and should be adopted in the primary cell culture, especially in fibroblast transplantation by injection.