Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

J Clin Microbiol

Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, California 94804, USA.

Published: March 2004

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC356861PMC
http://dx.doi.org/10.1128/JCM.42.3.1290-1293.2004DOI Listing

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