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From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in Species.
J Fungi (Basel)
December 2024
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
, a genus of fungi known for its fermentation capability and production of bioactive compounds, such as azaphilone pigments and Monacolin K, have received considerable attention because of their potential in biotechnological applications. Understanding the genetic basis of these metabolic pathways is crucial for optimizing the fermentation and enhancing the yield and quality of these products. However, spp.
View Article and Find Full Text PDFAncestral Sequence Reconstruction to Enable Biocatalytic Synthesis of Azaphilones.
J Am Chem Soc
November 2024
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
Biocatalysis can be powerful in organic synthesis but is often limited by enzymes' substrate scope and selectivity. Developing a biocatalytic step involves identifying an initial enzyme for the target reaction followed by optimization through rational design, directed evolution, or both. These steps are time consuming, resource-intensive, and require expertise beyond typical organic chemistry.
View Article and Find Full Text PDFUtilization of for the fermentative production of azaphilone dye in YEPB medium.
3 Biotech
November 2024
Department of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University, Lahore, 54000 Pakistan.
The current research focuses on the production and optimization of a natural yellowish-brown Azaphilone dye using . A variety of culture media were tested to ascertain the best conditions for dye synthesis. The formation of the yellowish-brown dye was confirmed by a color shift in the reaction mixture, and UV-Vis spectroscopy detected the dye at 450 nm.
View Article and Find Full Text PDFPleiotropically activation of azaphilone biosynthesis by overexpressing a pathway-specific transcription factor in marine-derived Aspergillus terreus RA2905.
Bioorg Chem
December 2024
Key Laboratory of Marine Drugs, the Ministry of Education of China, Institute of Evolution & Marine Biodiversity, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address:
The genome sequencing of Aspergillus terreus reveals that the vast number of predicted biosynthetic gene clusters have not reflected by the metabolic profile observed under conventional culture conditions. In this study, a silent azaphilone biosynthetic gene cluster was activated by overexpressing a pathway-specific transcription factor gene2642 in marine-derived fungus A. terreus RA2905.
View Article and Find Full Text PDFEffective enhancement of the ability of Monascus pilosus to produce lipid-lowering compound Monacolin K via perturbation of metabolic flux and histone acetylation modification.
Food Res Int
November 2024
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
Monacolin K (MK), also known as lovastatin, is a polyketide compound with the ability to reduce plasma cholesterol levels and many other bio-activities. Red yeast rice (also named Hongqu) rich in MK derived from Monascus fermentation has attracted widespread attention due to its excellent performance in reducing blood lipids. However, industrial Monascus fermentation suffers from the limitations such as low yield of MK, long fermentation period, and susceptibility to contamination.
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