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The MAP kinase kinase NtMEK2 is involved in tobacco pollen germination. | LitMetric

AI Article Synopsis

  • * The related SIPK MAP kinase is also present in pollen and is activated similarly when pollen hydrates.
  • * Inhibiting the kinase NtMEK2 resulted in reduced pollen germination, suggesting that both p45(Ntf4) and SIPK are crucial for this process.

Article Abstract

The tobacco ntf4 mitogen-activated protein (MAP) kinase gene (and its encoded protein p45(Ntf4)) is expressed at later stages of pollen maturation. We have found that the highly related MAP kinase SIPK is also expressed in pollen and, like p45(Ntf4), is activated upon pollen hydration. The MAP kinase kinase NtMEK2 activates SIPK, and here we show that it can also activate p45(Ntf4). In an attempt to inhibit the function of both MAP kinases simultaneously we constructed a loss-of-function mutant version of NtMEK2, which, in transient transformation assays, led to an inhibition of germination in the transformed pollen grains. These data indicate that NtMEK2, and by inference its substrates p45(Ntf4) and/or SIPK, are involved in pollen germination.

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Source
http://dx.doi.org/10.1016/S0014-5793(04)00076-6DOI Listing

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