[The studies on osteoblasts' mineralization and attachment affected by enamel matrix proteins].

Objective: Investigate the effects of enamel matrix proteins(EMPs) on the mineralization and attachment of osteoblasts, to explore the possible mechanism of alveolar bone regeneration induced by enamel matrix proteins(EMPs).

Methods: MC3T3-E1 osteoblasts were cultured in vitro and EMPs was added to medium in several concentrations. Cell's mineralizing ability was detected by Von-Kossa stain and attachment ability was detected by counting cells number.

Results: The results manifested that EMPs promote more mineral nodules formation (P<0.01). But MC3T3-E1 cells' attachment ability was not improved (P>0.05).

Conclusion: EMPs enhanced the mineralizing function of osteoblasts, had no effects on implanting on root surface of osteoblasts, which may play an important role in the regeneration of alveolar bone.