We report here on the successful extraction of human genomic DNA from a serum sample in a forensic case. The extracted DNA was successfully used for the identification of remains presumably immersed for more than three weeks for which the only comparison sample was a 250-microL serum aliquot kept frozen in a laboratory. The analysis made it possible to identify a second victim as the daughter of the first.
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J Clin Microbiol
January 2025
Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
Unlabelled: Rapid and accurate identification of cultured molds is important to determine clinical significance and therapeutic decision-making. Conventional mold identification uses phenotypic macroscopic and microscopic characterization; however, this can take days or weeks for colony maturity and definitive microscopic structure formation, be limited to genus-level identification, and be misidentified due to morphologic mimics or similarities between closely related species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) revolutionized bacterial and yeast identification but remains uncommon for molds in part because of limited reference libraries.
View Article and Find Full Text PDFJ Basic Microbiol
January 2025
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
One of the main difficulties in nanotechnology is the development of an environmentally friendly, successful method of producing nanoparticles from biological sources. Silver-doped zinc oxide nanoparticles (Ag-ZnO NPs), with antibacterial and antioxidant properties, were produced using Adiantum venustum extract as a green technique. Fresh A.
View Article and Find Full Text PDFBMJ Open
December 2024
School of Nutrition, Federal University of Bahia, Salvador, Brazil.
Introduction: Eating disorders can be irreversible and, in many cases, fatal. However, the symptoms full recovery is possible, and early diagnosis is one, of many, important factors for the success of treatment. In this sense, the screening of risk behaviours arises as a relevant alternative to improve the prognosis of patients.
View Article and Find Full Text PDFBMJ Support Palliat Care
December 2024
Health Research Institute, School of Design, and Confirm Smart Manufacturing Centre, University of Limerick, Limerick, Ireland
Background: Three-dimensional printing (3DP) enables the production of highly customised, cost-efficient devices in a relatively short time, which can be particularly valuable to clinicians treating patients with palliative care intent who are in need of timely and effective solutions in the management of their patients' specific needs, including the relief of distressing symptoms.
Method: Four online databases were searched for articles published by December 2020 that described studies using 3DP in palliative care. The fields of application, and the relevant clinical and technological data were extracted and analysed.
BMC Health Serv Res
January 2025
Department of Speech and Language Pathology, School of Rehabilitation Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
Introduction: Communication disorders are one of the most common disorders that, if not treated in childhood, can cause many social, educational, and psychological problems in adulthood. One of the technologies that can be helpful in these disorders is mobile health (m-Health) technology. This study aims to examine the attitude and willingness to use this technology and compare the advantages and challenges of this technology and face-to-face treatment from the perspective of patients.
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