Adeninyl POM was prepared using solid-phase peptide chemistry and shown to exhibit higher affinity for complementary DNA and RNA than the corresponding adeninyl PNA.

Download full-text PDF

Source
http://dx.doi.org/10.1039/b315768gDOI Listing

Publication Analysis

Top Keywords

nucleic acid
4
acid binding
4
binding properties
4
properties thyminyl
4
thyminyl adeninyl
4
adeninyl pyrrolidine-amide
4
pyrrolidine-amide oligonucleotide
4
oligonucleotide mimics
4
mimics pom
4
pom adeninyl
4

Similar Publications

The future of cell-free synthetic biology.

Biotechnol Notes

November 2024

Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

Cell-free synthetic biology aims at the targeted replication, design, and modification of life processes in open systems by breaking free of constraints such as cell membrane barriers and living cell growth. The beginnings of this systematized technology, which took place in the last century, were used to explore the secrets of life. Currently, with its easy integration with other technologies or disciplines, cell-free synthetic biology is developing into a powerful and effective means of understanding, exploiting, and extending the structure and function of natural living systems.

View Article and Find Full Text PDF

Anti-gene oligonucleotide clamps invade dsDNA and downregulate expression.

Mol Ther Nucleic Acids

December 2024

Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred Nobels Allé 8, 14152 Huddinge, Stockholm, Sweden.

Anti-gene oligonucleotides belong to a group of therapeutic compounds, which, in contrast to antisense oligonucleotides, bind to DNA. Clamp anti-gene oligonucleotides bind through a double-stranded invasion mechanism. With two arms connected by a linker, they hybridize to one of the DNA strands forming Watson-Crick and Hoogsteen hydrogen bonds.

View Article and Find Full Text PDF

Biological polyamines, such as spermine, spermidine, and putrescine, are abundant intracellular compounds mostly bound to nucleic acids. Due to their nucleophilic nature, polyamines easily react with apurinic/apyrimidinic (AP) sites, DNA lesions that are constantly formed in DNA by spontaneous base loss and as intermediates of base excision repair. A covalent intermediate is formed, promoting DNA strand cleavage at the AP site, and is later hydrolyzed regenerating the polyamine.

View Article and Find Full Text PDF

This study investigated the role of brain-derived neurotrophic factor (BDNF) in patients with degenerative lumbar stenosis, focusing on its expression and correlation with pain intensity. The study examined 96 patients with lumbar stenosis and 85 control participants. BDNF levels in the yellow ligamentum flavum were measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), and western blot analysis.

View Article and Find Full Text PDF

Polymers for mRNA Delivery.

Wiley Interdiscip Rev Nanomed Nanobiotechnol

January 2025

Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, China.

mRNA delivery has emerged as a transformative approach in biotechnology and medicine, offering a versatile platform for the development of novel therapeutics. Unlike traditional small molecule drugs or protein-based biologics, mRNA therapeutics have the unique ability to direct cells to generate therapeutic proteins, allowing for precise modulation of biological processes. The delivery of mRNA into target cells is a critical step in realizing the therapeutic potential of this technology.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!