The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 degrees C. Therefore, cryopreservation of oocytes by vitrification--especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications--seems the most appropriate method.
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| http://dx.doi.org/10.1051/rnd:2003024 | DOI Listing |
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