Objective: To observe the influence of different tocopherol isoforms on oxidized low density lipoprotein (oxLDL) or recombinant human C-reactive protein (rhCRP)-induced expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) and to investigate the potential mechanisms and effects of different tocopherols on atherosclerosis.
Methods: Cultured HUVECs were incubated with oxLDL, oxLDL + alpha-tocopherol, oxLDL + gamma-tocopherol, oxLDL + mixed-tocopherols, rhCRP, rhCRP + alpha-tocopherol, rhCRP + gamma-tocopherol rhCRP + mixed-tocopherols for 24 hours, respectively. The ICAM-1 expressions of protein and mRNA were detected by cell enzyme linked immunosorbent assay (ELISA), flow cytometric technique and RT-PCR.
Results: Incubation of HUVECs with oxLDL or rhCRP for 24 hours significantly increased ICAM-1 expressions of proteins and mRNA. The different tocopherols inhibited oxLDL-induced ICAM-1 expression in HUVECs in a concentration-dependent manner (50-200 micromol/L) and mixed-tocopherols were more potent than alpha-tocopherol or gamma-tocopherol alone. However, rhCRP-induced ICAM-1 expression in HUVECs was not inhibited by tocopherols.
Conclusion: The different tocopherols inhibited oxLDL-induced ICAM-1 expression in HUVECs and mixed-tocopherols were more potent than alpha-tocopherol or gamma-tocopherol alone, which may be important for the beneficial effects of tocopherols on atherosclerosis and cardiovascular disease.
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Alzheimers Dement
December 2024
UK Dementia Research Institute, University of Edinburgh, Edinburgh, United Kingdom.
Background: Small vessel disease (SVD) is a disorder of the brain's microvessels and a common cause of dementia and stroke. Evidence links normal ageing features to SVD progression, involving endothelial activation, pericyte dysfunction, BBB failure, and microglia response. Here, we aim to examine this relationship through a series of translational investigations.
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December 2024
Alzheimer's Center at Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Background: Neurofibrillary tangles formed by hyperphosphorylated tau aggregates in the brain are one of the classical hallmarks of Alzheimer's Disease (AD). Tau aggregates have been shown to elicit cytotoxicity, leading to overall neuronal loss and cognitive decline in AD. These aggregates can be transmitted from neurons and glial cells to other brain cells through a process known as tau spreading, and ultimately reach the endothelial cells (ECs) lining the vessel walls, thus, causing dysfunction of the neurovascular unit (NVU), a complex multicellular system surrounding brain vessels.
View Article and Find Full Text PDFFront Mol Biosci
December 2024
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, GA, United States.
Introduction: Sickle cell disease (SCD) is a genetic blood disorder caused by a mutation in the HBB gene, which encodes the beta-globin subunit of hemoglobin. This mutation leads to the production of abnormal hemoglobin S (HbS), causing red blood cells to deform into a sickle shape. These deformed cells can block blood flow, leading to complications like chronic hemolysis, anemia, severe pain episodes, and organ damage.
View Article and Find Full Text PDFImmunopharmacol Immunotoxicol
January 2025
Department of Regenerative Dental Medicine, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Tokushima, Japan.
Objectives: Periodontal disease is a chronic inflammatory disease caused by periodontopathogenic bacteria, and its progression leads to periodontal tissue destruction and tooth loss. Zerumbone is a bioactive substance found in ginger () and is known to have bioactive effects such as anticancer effects, but there have been no attempts to use it for periodontitis treatment. In addition, there have been no reports examining its effects on periodontal tissue component cells.
View Article and Find Full Text PDFJ Virol
December 2024
Laboratory of Virology, Regional Centre for Biotechnology, National Capital Region Biotechnology Science Cluster, Faridabad, Haryana, India.
Extracellular vesicles (EVs) emerged as critical contributors to the pathogenesis of vascular endothelial barrier dysfunction during the inflammatory response to infection. However, the contribution of circulating EVs to modifying endothelial function during dengue virus infection remains unclear. In this study, we showed that severe dengue patients' plasma-derived EV (SD-EV) were found to carry elevated levels of different protein cargos, e.
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