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Purpose: The objective was to use cyclic tensile loading to compare the gap formation at suture site of three different suture materials to repair bovine radial meniscal tears: (1) polyglactin sutures, (2) tough adhesive puncture sealing (TAPS) sutures and (3) ultra-high molecular weight polyethylene (UHMWPE) sutures.
Methods: Twelve ex vivo bovine knees were dissected to retrieve the menisci. Complete radial tears were performed on 24 menisci, which were then separated into three groups and repaired using either pristine 2-0 polyglactin sutures, TAPS sutures (2-0 polyglactin sutures coated with adhesive chitosan/alginate hydrogels) or 2-0 UHMWPE sutures with a single stitch.
The translational potential of inflammation-induced skin blister human models in exploring the pathogenesis of periodontitis and its systemic health implications.
Front Immunol
December 2024
Periodontology Unit, UCL Eastman Dental Institute, University College London, London, United Kingdom.
Periodontitis is a highly prevalent chronic disease. Despite decades of extensive research on the topic, a complete understanding of its immunopathogenesis, especially when linked to other inflammatory comorbidities, is lacking. human and animal experiments have shown the host inflammatory response's crucial role in both the disease's onset and its systemic implications.
View Article and Find Full Text PDFThree-dimensional models: from cell culture to Patient-Derived Organoid and its application to future liposarcoma research.
Oncol Res
December 2024
Department of Surgery, Division of Surgical Oncology, The James Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
Liposarcoma is one of the most common soft tissue sarcomas, however, its occurrence rate is still rare compared to other cancers. Due to its rarity, experiments are an essential approach to elucidate liposarcoma pathobiology. Conventional cell culture-based research (2D cell culture) is still playing a pivotal role, while several shortcomings have been recently under discussion.
View Article and Find Full Text PDFImmunofluorescence for Detection of TOR Kinase Activity In Situ in Photosynthetic Organisms.
Bio Protoc
December 2024
Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC) and FIBA, Vieytes 3103, Mar del Plata, Argentina.
The target of rapamycin (TOR) is a central hub kinase that promotes growth and development in all eukaryote cells. TOR induces protein synthesis through the phosphorylation of the S6 kinase (S6K), which, in turn, phosphorylates ribosomal S6 protein (RPS6) increasing this anabolic process. Therefore, S6K and RPS6 phosphorylation are generally used as readouts of TOR activity.
View Article and Find Full Text PDFEnhanced effects of levan hydrogels and bovine grafts on guided bone regeneration: In-vitro and in-vivo analysis.
Int J Biol Macromol
December 2024
Bioengineering, Faculty of Engineering, Marmara University, Istanbul, Turkey.
This study was performed to evaluate the regenerative capabilities of levan hydrogels when combined with conventional bone graft materials (Bio-Oss®) in guided bone regeneration (GBR). With the growing interest in the application of levan polysaccharide for regenerative purposes over the last decade, a noticeable gap in in-vivo validations remains. This research therefore fills this gap by utilizing the cytocompatibility and cell proliferation potential of levan hydrogels and marks a preliminary effort in its use in combination with Bio-Oss® for bone regeneration, which was examined both in-vitro and in-vivo for the first time.
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