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Characterizing a lethal CAG-ACE2 transgenic mouse model for SARS-CoV-2 infection using Cas9-enhanced nanopore sequencing.
Transgenic Res
October 2024
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia, 630090.
The SARS-CoV-2 pandemic has underscored the necessity for functional transgenic animal models for testing. Mouse lines with overexpression of the human receptor ACE2 serve as the common animal model to study COVID-19 infection. Overexpression of ACE2 under a strong ubiquitous promoter facilitates convenient and sensitive testing of COVID-19 pathology.
View Article and Find Full Text PDFShape of the first mitotic spindles impacts multinucleation in human embryos.
Nat Commun
June 2024
Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
During human embryonic development, early cleavage-stage embryos are more susceptible to errors. Studies have shown that many problems occur during the first mitosis, such as direct cleavage, chromosome segregation errors, and multinucleation. However, the mechanisms whereby these errors occur during the first mitosis in human embryos remain unknown.
View Article and Find Full Text PDFCRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation.
J Vis Exp
March 2024
Animal Modeling Core, University of Missouri; Comparative Medicine Program, University of Missouri; Rat Resource and Research Center, University of Missouri; Department of Veterinary Pathobiology, University of Missouri.
Genome editing technology is widely used to produce genetically modified animals, including rats. Cytoplasmic or pronuclear injection of DNA repair templates and CRISPR-Cas reagents is the most common delivery method into embryos. However, this type of micromanipulation necessitates access to specialized equipment, is laborious, and requires a certain level of technical skill.
View Article and Find Full Text PDFβ-glucosidase, driven by porcine transthyretin promoter, specific expression in the liver of transgenic mice.
Anim Sci J
December 2023
Key Laboratory of Swine Genetics and Breeding of the Agricultural Ministry, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
Under the background of food security, using non-grain feed instead of corn-soybean-based feed is an effective measure to alleviate the food-feed competition. While, non-grain feeds are often rich in fiber, which cannot be digested by non-ruminants. Producing heterologous enzymes in non-ruminants to improve cellulose utilization rate is a new research strategy by transgenic technology.
View Article and Find Full Text PDFHow genome editing changed the world of large animal research.
Front Genome Ed
October 2023
Chair of Livestock Biotechnology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany.
The first genetically modified large animals were developed in 1985 by microinjection to increase the growth of agricultural livestock such as pigs. Since then, it has been a difficult trail due to the lack of genetic tools. Although methods and technologies were developed quickly for the main experimental mammal, the mouse, e.
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