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Lack of technical specificity in the molecular diagnosis of toxoplasmosis. | LitMetric

Lack of technical specificity in the molecular diagnosis of toxoplasmosis.

Trans R Soc Trop Med Hyg

Instituto Oswaldo Cruz, Laboratório de Doenças Parasitarias, Departamento de Medicina Tropical, Av. Brazil 4365, 21045-900-Manguinhos, Rio de Janeiro, Brazil.

Published: February 2004

The polymerase chain reaction amplification of a fragment of the B1 gene of Toxoplasma gondii coupled to hybridization was performed in 42 patients from Rio de Janeiro, Brazil. The results showed 50% of positivity in the IgM positive toxoplasmosis group, and 12.5% in the positive IgG and negative IgM individuals. The data presented here revealed a lack of specificity of the molecular approach, clearly indicating that the primers used may co-amplify human sequences.

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http://dx.doi.org/10.1016/s0035-9203(03)00012-9DOI Listing

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